IFSS Human and Animal Food Laboratory

Entry (Program Specific)

Chemistry

Content Area: Basic Separation / Extraction Techniques

Description: The theory and application related to current techniques to extract compounds from test portions.

Topic Areas:

  • Description: Principles of separation and extraction.

    Terminal Competency:

    Describe the principles for maximizing extraction efficiency.

    Enabling Competencies:

    1. Describe the role of partition efficiency in maximizing analyte extraction.

    2. Describe how solute/solvent interactions impact extraction efficiency.

    3. Describe the effect of pH on an extraction.

    4. Discuss how column capacity impacts analyte recovery.

    To see a set of behavioral anchors, which provide further detail about the enabling competencies and can be used for assessment against the enabling competencies, contact IFPTI.

  • Description: Techniques for the separation of chemical analytes from a test portion.

    Terminal Competency:

    Describe techniques to separate an analyte of interest from a test portion.

    Enabling Competencies:

    1. Describe the use of solvents to separate an analyte from a matrix.

    2. Describe matrix destruction techniques.

    3. Describe separation of analyte using preparatory chromatography techniques.

    To see a set of behavioral anchors, which provide further detail about the enabling competencies and can be used for assessment against the enabling competencies, contact IFPTI.

  • Description: Equipment used for analyte extraction.

    Terminal Competency:

    Identify equipment used for analyte extraction.

    Enabling Competencies:

    1. List equipment/specialized labware for analyte extraction procedures.

    2. Identify instrumentation used for analyte extraction.

    To see a set of behavioral anchors, which provide further detail about the enabling competencies and can be used for assessment against the enabling competencies, contact IFPTI.

  • Description: Considerations that impact analyte extraction.

    Terminal Competency:

    Explain considerations that can affect analyte recovery.

    Enabling Competencies:

    1. Explain ways to resolve incomplete phase separation.

    2. Describe how to minimize contaminants/contamination.

    3. Explain how analyst technique can impact analyte recovery.

    4. Describe how to minimize analyte loss.

    To see a set of behavioral anchors, which provide further detail about the enabling competencies and can be used for assessment against the enabling competencies, contact IFPTI.

Learning Resources:

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Assessments:

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Basic Chromatography

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Basic Mass Spectrometry